Potential of hydrocabonoclastik bacteria for dergrading diesel oil in the port of Gresik

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Omar, Mohamed Abdirahman (2023) Potential of hydrocabonoclastik bacteria for dergrading diesel oil in the port of Gresik. Undergraduate thesis, UIN Sunan Ampel Surabaya.

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Abstract

The high density of traffic at the port of Gresik, located in the Madura Strait, raises a new problem, namely the increasing number of ships, which increases the capacity of refueling diesel oil on ships, resulting in a volume of waste containing oil that tends to increase. In conditions of overcapacity and proneness to accidents, shipping will be at risk of an oil spill disaster from the ship's cargo. Alternative countermeasures are appropriate and do not disturb the environment by using hydrocarbonoclastic bacteria that can degrade diesel oil which is called bioremediation method. The purpose of this research is to find out which bacteria have the potential to degrade diesel oil in Gresik Port and to determine the ability of these bacterial isolates to degrade diesel oil. In laboratory experiments, a diesel oil-degrading bacterial consortium was used to biodegrade diesel oil. The bacterial consortium was prepared in liquid medium for laboratory experiments, and the water sample was taken at the port of Gresik. This study uses a purposive sampling method in seawater sampling and uses experimental methods in the laboratory for bacterial isolation, oil degradation, characterization of bacterial observations, and biodegradation tests on diesel oil. The results of research on the waters of the Port of Gresik were obtained 4 isolates of diesel oil degrading bacteria, where isolates are found bacteria, namely the genus Marinobacter xestospongiae. The results of the identification of bacteria capable of degrading diesel oil significantly are a consortium (mixed isolates in the genus Marinobacter xestospongiae) with a biodegradation percentage of 92.25%.